4t1 breast cancer cell lines  (ATCC)

Journal: Cancer Research

Article Title: ACSL5 Mediates Adaptation to the Palmitic Acid–Enriched Pulmonary Microenvironment to Enhance Metastatic Breast Cancer Cell Survival and Lung Metastasis

doi: 10.1158/0008-5472.CAN-25-0866

Figure Lengend Snippet: ACSL5 is highly expressed in lung metastatic breast cancer cells and fostered lung metastasis. A, Eight metabolism-related genes are commonly upregulated in lung metastatic breast cancer cells. B, Western blotting was used to evaluate ACSL5 protein levels in primary tumor cells (PRI) and organ-specific derivatives (left), as well as in the lung metastatic derivatives (right) of MDA-MB-231. LM1, the first generation of lung metastasis; LM2, the second generation of lung metastasis; LM3, the third generation of lung metastasis, breast cancer cells with lung-preferential metastasis. C and D, Representative images ( C ) and quantification ( D ) of IHC of ACSL5 protein levels in primary tumors (PRI) and lung metastases of patients with breast cancer ( n = 7). E, ACSL5 mRNA levels in lung, liver, brain, and bone metastases of patients with breast cancer ( GSE14020 ). F, Kaplan–Meier survival curves for overall survival of patients with breast cancer with low and high ACSL5 mRNA levels ( GSE14020 ). G, Representative images of BLI. BALB/c nude mice were injected with MDA-MB-231 and HCC1806 cells. BALB/c mice were injected with 4T1 cells. All data represent the mean ± SD. Student t test ( D ), Kruskal–Wallis test ( E ) and log-rank test ( F ) were utilized. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Article Snippet: Triple-negative breast cancer cell lines 4T1, MDA-MB-231, and HCC1806 and mouse alveolar epithelial cell line MLE-12 were purchased from the ATCC.

Techniques: Western Blot, Injection